Bethyl Laboratories
Rabbit anti-WDR77/MEP50 Antibody Affinity Purified
Primary
Catalog #A301-562A
TargetWDR77/MEP50
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
WDR77/MEP50 is a component of the methylosome, a large protein arginine methyltransferase complex that modifies specific arginines to dimethylarginines in splicesomal Sm proteins. WDR77/MEP50 associates with the methyltransferase JBP1 (PRMT5) and is important for methylosome activity. WDR77/MEP50 has also been shown to associate with the polycomb group protein, SUZ12, and histone H2A. In this context, WDR77/MEP50 is proposed to function as an adaptor between PRMT5 and its substrate H2A to regulate transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 292 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetWDR77/MEP50
Antigen Species
Human
Gene ID79084
Gene Symbol
WDR77
Gene Name
WD repeat domain 77
Uniprot IDQ9BQA1
Protein Name
Methylosome protein 50
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR77/MEP50 immobilized on solid support.
The epitope recognized by A301-562A maps to a region between residue 292 and 342 of human WD repeat domain 77 (methylosome protein 50) using the numbering given in entry NP_077007.1 (GeneID 79084).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-562A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-562A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-562AOpen
Citations / Publications
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