Bethyl Laboratories
Rabbit anti-WDR77/MEP50 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00338
TargetWDR77/MEP50
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF
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Product Description
WDR77/MEP50 is a component of the methylosome, a large protein arginine methyltransferase complex that modifies specific arginines to dimethylarginines in splicesomal Sm proteins. WDR77/MEP50 associates with the methyltransferase JBP1 (PRMT5) and is important for methylosome activity. WDR77/MEP50 has also been shown to associate with the polycomb group protein, SUZ12, and histone H2A. In this context, WDR77/MEP50 is proposed to function as an adaptor between PRMT5 and its substrate H2A to regulate transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 292 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
50 µg/ml
Target Identity
TargetWDR77/MEP50
Antigen Species
Human
Gene ID79084
Gene Symbol
WDR77
Gene Name
WD repeat domain 77
Uniprot IDQ9BQA1
Protein Name
Methylosome protein 50
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDR77/MEP50 immobilized on solid support.
The epitope recognized by IHC-00338 maps to a region between residue 292 and 342 of human WD repeat domain 77 (methylosome protein 50) using the numbering given in entry NP_077007.1 (GeneID 79084).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00338Open
Product DatasheetPDF · IHC-00338 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00338 · Lot 1 (current lot)Open
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