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Rabbit anti-WTAP Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
WTAP
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 250 and 300
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.

The epitope recognized by A301-435A-T maps to a region between residue 250 and 300 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.

The epitope recognized by A301-435A maps to a region between residue 250 and 300 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Wilms’ tumor 1 associated protein (WTAP) is a protein found to associate with the Wilms’ tumor suppressor (WT1). WTAP appears to play a role in cell proliferation and regulation of the G2/M transition. WTAP has been suggested to influence these processes via a function in mRNA stabilization and mRNA splicing.

9589
WTAP
WT1 associated protein
Q15007
Pre-mRNA-splicing regulator WTAP

Alternate Names

female-lethal(2)D homolog; hFL(2)D; Mum2; PNAS-132; pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; WT1-associated protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.