Bethyl Laboratories
Rabbit anti-WTAP Antibody Affinity Purified
Primary
Catalog #A301-435A
TargetWTAP
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Wilms’ tumor 1 associated protein (WTAP) is a protein found to associate with the Wilms’ tumor suppressor (WT1). WTAP appears to play a role in cell proliferation and regulation of the G2/M transition. WTAP has been suggested to influence these processes via a function in mRNA stabilization and mRNA splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWTAP
Antigen Species
Human
Gene ID9589
Gene Symbol
WTAP
Gene Name
Wilms tumor 1 associated protein
Uniprot IDQ15007
Protein Name
Pre-mRNA-splicing regulator WTAP
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.
The epitope recognized by A301-435A maps to a region between residue 250 and 300 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-435A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-435A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-435AOpen
Citations / Publications
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