/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-WTAP Antibody
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Primary Antibodies  

Rabbit anti-WTAP Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (3)
Target:

WTAP

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 346 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

X. laevis,

X. tropicalis

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.

The epitope recognized by A301-436A maps to a region between residue 346 and 396 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.

The epitope recognized by A301-436A-T maps to a region between residue 346 and 396 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).

Country of Origin

USA

Additional Product Information

Wilms’ tumor 1 associated protein (WTAP) is a protein found to associate with the Wilms’ tumor suppressor (WT1). WTAP appears to play a role in cell proliferation and regulation of the G2/M transition. WTAP has been suggested to influence these processes via a function in mRNA stabilization and mRNA splicing.

9589

WTAP

WT1 associated protein

Q15007

Pre-mRNA-splicing regulator WTAP

Alternate Names

female-lethal(2)D homolog; hFL(2)D; Mum2; PNAS-132; pre-mRNA-splicing regulator WTAP; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; WT1-associated protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.