Bethyl Laboratories
Rabbit anti-WTAP IHC Antibody Affinity Purified
Primary
Catalog #IHC-00368
TargetWTAP
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
Wilms’ tumor 1 associated protein (WTAP) is a protein found to associate with the Wilms’ tumor suppressor (WT1). WTAP appears to play a role in cell proliferation and regulation of the G2/M transition. WTAP has been suggested to influence these processes via a function in mRNA stabilization and mRNA splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 346 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetWTAP
Antigen Species
Human
Gene ID9589
Gene Symbol
WTAP
Gene Name
WT1 associated protein
Uniprot IDQ15007
Protein Name
Pre-mRNA-splicing regulator WTAP
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WTAP immobilized on solid support.
The epitope recognized by IHC-00368 maps to a region between residue 346 and 396 of human Wilms tumor 1 associated protein using the numbering given in entry NP_004897.2 (GeneID 9589).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00368Open
Product DatasheetPDF · IHC-00368 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00368 · Lot 1 (current lot)Open
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