Bethyl Laboratories
Rabbit anti-WWP1 Antibody Affinity Purified
Primary
Catalog #A302-950A
TargetWWP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
WW domain-containing protein 1 (WWP1) functions as an E3 ubiquitin-protein ligase. WWP1 ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. WWP1 also ubiquitinates and promotes degradation of TGFBR1; TGFBR1 ubiquitination is enhanced by SMAD7, and WWP1 also ubiquitinates SMAD7 as well as SMAD6. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9H0M0].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWWP1
Antigen Species
Human
Gene ID11059
Gene Symbol
WWP1
Gene Name
WW domain containing E3 ubiquitin protein ligase 1
Uniprot IDQ9H0M0
Protein Name
NEDD4-like E3 ubiquitin-protein ligase WWP1
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WWP1 immobilized on solid support.
The epitope recognized by A302-950A maps to a region between residue 300 and 350 of human WW Domain Containing E3 Ubiquitin Protein Ligase 1 using the numbering given in entry NP_008944.1 (GeneID 11059).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-950A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-950A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-950AOpen
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