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Primary Antibodies  

Rabbit anti-XLF Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (2)
Target:

XLF

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 200 and 250

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

XRCC4-like factor (XLF) functions in DNA nonhomologous end joining (NHEJ) in double-strand break repair and immune system V(D)J recombination. Mutations of XLF in humans have been documented to result in immunodeficiency, growth retardation, and microcephaly. The yeast of homolog of XLF is Nej1. XLF has been shown to interact with the XRCC4 x DNA-ligase IV complex and is implicated in the DNA religation step of the NHEJ pathway.

79840

NHEJ1

non-homologous end joining factor 1

Q9H9Q4

Non-homologous end-joining factor 1

Alternate Names

non-homologous end-joining factor 1; nonhomologous end-joining factor 1; protein cernunnos; XLF; XRCC4-like factor

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.