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Rabbit anti-XLF Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
XLF
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 200 and 250
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to XLF immobilized on solid support.

The epitope recognized by A300-729A-T maps to a region between residue 200 and 250 of human XRCC4-Like Factor using the numbering given in entry NP_079058.1 (GeneID 79840).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

XRCC4-like factor (XLF) functions in DNA nonhomologous end joining (NHEJ) in double-strand break repair and immune system V(D)J recombination. Mutations of XLF in humans have been documented to result in immunodeficiency, growth retardation, and microcephaly. The yeast of homolog of XLF is Nej1. XLF has been shown to interact with the XRCC4 x DNA-ligase IV complex and is implicated in the DNA religation step of the NHEJ pathway.

79840

NHEJ1

non-homologous end joining factor 1

Q9H9Q4

Non-homologous end-joining factor 1

Alternate Names

non-homologous end-joining factor 1, nonhomologous end-joining factor 1, protein cernunnos, XLF, XRCC4-like factor

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE.