Bethyl Laboratories
Rabbit anti-XPC Antibody Affinity Purified
Primary
Catalog #A301-122A
TargetXPC
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Defects in XPC are a cause of xeroderma pigmentosum complementation group C, a rare autosomal recessive diseased characterized by hypersensitivity to sunlight and an elevated risk of skin cancer on sun-exposed areas. XPC is involved in nucleotide excision repair and performs its function as part of a heterodimeric complex with HHR23B. XPC has been found to play a role in the early stages of repair that involve incising damaged DNA.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 890 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetXPC
Antigen Species
Human
Gene ID7508
Gene Symbol
XPC
Gene Name
XPC complex subunit, DNA damage recognition and repair factor
Uniprot IDQ01831
Protein Name
DNA repair protein complementing XP-C cells
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to XPC immobilized on solid support.
The epitope recognized by A301-122A maps to a region between residue 890 and 940 of human xeroderma pigmentosum, complementation group C using the numbering given in entry NP_004619.2 (GeneID 7508).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-122A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-122A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-122AOpen
Citations / Publications
Services
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