Bethyl Laboratories
Rabbit anti-YAP1 Antibody Affinity Purified
Primary
Catalog #A302-309A
TargetYAP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Yes-associated protein 1 (YAP1) is the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction [taken from NCBI GeneID:10413].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 425 and 475
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetYAP1
Antigen Species
Human
Gene ID10413
Gene Symbol
YAP1
Gene Name
Yes associated protein 1
Uniprot IDP46937
Protein Name
Transcriptional coactivator YAP1
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to YAP1 immobilized on solid support.
The epitope recognized by A302-309A maps to a region between residue 350 and 400 of human Yes-associated protein 1 using the numbering given in entry NP_006097.1 (GeneID 10413).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-309A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-309A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-309AOpen
Citations / Publications
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