Bethyl Laboratories
Rabbit anti-YTHDF2 Recombinant Monoclonal Antibody [BLR297M]
Primary
Catalog #A700-297
TargetYTHDF2
HostRabbit
CloneBLR297M
ReactivityMouse, Human
ApplicationFlow Cyt, WB
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Product Description
YTHDF2 is a member of the YTH-domain containing (YT521-B homology) superfamily which also contains YTHDF1 and YTHDF3. These proteins share a conserved C-terminal YTH domain that specifically interacts with N6-methyladenosine (m6A) modified RNA. The m6A modification is present at internal sites of mRNAs and some non-coding RNAs and plays an important role in mRNA splicing, processing, and stability. YTHDF2 binding appears to promote mRNA degradation, but has minimal effect on translation efficiency. [adapted from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y5A9].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR297M
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
500 µg/ml
Target Identity
TargetYTHDF2
Antigen Species
Human
Gene ID51441
Gene Symbol
YTHDF2
Gene Name
YTH N6-methyladenosine RNA binding protein 2
Uniprot IDQ9Y5A9
Protein Name
YTH domain-containing family protein 2
Product Information
Applications
Flow Cyt, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 300 and 350 of human YTH domain-containing family protein 2 using the numbering given in entry NP_057342.2 (Gene ID 51441).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:1000
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Documents
Product DatasheetPDF · A700-297 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-297 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-297 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-297Open
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