Bethyl Laboratories
Rabbit anti-YY1 Antibody Affinity Purified
Primary
Catalog #A302-778A
TargetYY1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
YY1 (ying and yang 1) is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetYY1
Antigen Species
Human
Gene ID7528
Gene Symbol
YY1
Gene Name
YY1 transcription factor
Uniprot IDP25490
Protein Name
Transcriptional repressor protein YY1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to YY1 immobilized on solid support.
The epitope recognized by A302-778A maps to a region between residue 1 and 50 of human Yin and Yang 1 Protein using the numbering given in entry NP_003394.1 (GeneID 7528).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-778A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-778A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-778AOpen
Citations / Publications
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