Bethyl Laboratories
Rabbit anti-ZAP70 Antibody Affinity Purified
Primary
Catalog #A301-818A
TargetZAP70
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 569 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetZAP70
Antigen Species
Human
Gene ID7535
Gene Symbol
ZAP70
Gene Name
zeta chain of T cell receptor associated protein kinase 70
Uniprot IDP43403
Protein Name
Tyrosine-protein kinase ZAP-70
Alternate Names
70 kDa zeta-associated protein • 70 kDa zeta-chain associated protein • ADMIO2 • IMD48 • SRK • STCD • STD • syk-related tyrosine kinase • tyrosine-protein kinase ZAP-70 • TZK • ZAP-70 • zeta chain of T cell receptor associated protein kinase 70kDa • zeta-chain (TCR) associated protein kinase 70kDa • zeta-chain associated protein kinase, 70kD
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZAP70 immobilized on solid support.
The epitope recognized by A301-818A maps to a region between residue 564 and 614 of human zeta-chain (TCR) associated protein kinase 70kDa using the numbering given in entry NP_001070.2 (GeneID 7535).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-818A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-818A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-818AOpen
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