Bethyl Laboratories
Rabbit anti-ZBTB7/FBI-1 Antibody Affinity Purified
Primary
Catalog #A300-548A
TargetZBTB7/FBI-1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Zinc-finger and BTB domain-containing protein 7A (ZBTB7A) binds IST (inducer of short transcript) DNA elements in the HIV promoter to facilitate short transcript synthesis. ZBTB7A identified as LRF also interacts with the LAZ-3/BCL-6 oncoprotein. Because ZBTB7A also contains a POZ domain it may serve a function in chromatin remodeling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetZBTB7/FBI-1
Antigen Species
Human
Gene ID51341
Gene Symbol
ZBTB7A
Gene Name
zinc finger and BTB domain containing 7A
Uniprot IDO95365
Protein Name
Zinc finger and BTB domain-containing protein 7A
Alternate Names
factor binding IST protein 1 • factor that binds to inducer of short transcripts protein 1 • FBI1 • FBI-1 • HIV-1 1st-binding protein 1 • HIV-1 inducer of short transcripts binding protein • leukemia/lymphoma-related factor • LRF • lymphoma related factor • POK erythroid myeloid ontogenic factor • pokemon • Pokemon 1 • POZ and Krueppel erythroid myeloid ontogenic factor • TIP21 • TTF-I-interacting peptide 21 • ZBTB7 • zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein • zinc finger and BTB domain-containing protein 7A • zinc finger protein 857A • ZNF857A
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZBTB7/FBI-1 immobilized on solid support.
The epitope recognized by A300-548A maps to a region between residues 200 and 250 of human ZBTB7 (Factor Binding IST protein 1) using the numbering given in entry NP_056982.1 (GeneID 51341).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-548A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-548A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-548AOpen
Citations / Publications
Services
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