Bethyl Laboratories
Rabbit anti-ZBTB7/FBI-1 Antibody Affinity Purified
Primary
Catalog #A300-549A
TargetZBTB7/FBI-1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Zinc-finger and BTB domain-containing protein 7A (ZBTB7A) binds IST (inducer of short transcript) DNA elements in the HIV promoter to facilitate short transcript synthesis. ZBTB7A identified as LRF also interacts with the LAZ-3/BCL-6 oncoprotein. Because ZBTB7A also contains a POZ domain it may serve a function in chromatin remodeling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 534 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetZBTB7/FBI-1
Antigen Species
Human
Gene ID51341
Gene Symbol
ZBTB7A
Gene Name
zinc finger and BTB domain containing 7A
Uniprot IDO95365
Protein Name
Zinc finger and BTB domain-containing protein 7A
Alternate Names
factor binding IST protein 1 • factor that binds to inducer of short transcripts protein 1 • FBI1 • FBI-1 • HIV-1 1st-binding protein 1 • HIV-1 inducer of short transcripts binding protein • leukemia/lymphoma-related factor • LRF • lymphoma related factor • POK erythroid myeloid ontogenic factor • pokemon • Pokemon 1 • POZ and Krueppel erythroid myeloid ontogenic factor • TIP21 • TTF-I-interacting peptide 21 • ZBTB7 • zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein • zinc finger and BTB domain-containing protein 7A • zinc finger protein 857A • ZNF857A
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZBTB7/FBI-1 immobilized on solid support.
The epitope recognized by A300-549A maps to a region between residue 525 and the C-terminus (residue 584) of human ZBTB7 (Factor Binding IST protein 1) using the numbering given in entry NP_056982.1 (GeneID 51341).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-549A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-549A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-549AOpen
Citations / Publications
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