Bethyl Laboratories
Rabbit anti-ZC3H8 Antibody Affinity Purified
Primary
Catalog #A303-089A
TargetZC3H8
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Zinc-finger CCCH domain-containing protein 8 (ZC3H8) acts as a transcriptional repressor of the GATA3 promoter. It is a sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (by similarity). ZC3H8 induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q8N5P1].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetZC3H8
Antigen Species
Human
Gene ID84524
Gene Symbol
ZC3H8
Gene Name
zinc finger CCCH-type containing 8
Uniprot IDQ8N5P1
Protein Name
Zinc finger CCCH domain-containing protein 8
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZC3H8 immobilized on solid support.
The epitope recognized by A303-089A maps to a region between residue 1 and 50 of human Znc Finger CCCH-type Containing 8 using the numbering given in entry NP_115883.2 (GeneID 84524).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-089A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-089A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-089AOpen
Citations / Publications
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