Bethyl Laboratories
Rabbit anti-ZEB1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00419
TargetZEB1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). Mutations in the ZEB1 gene are associated with posterior corneal dystrophy type 3 (PPC3) a rare disorder characterized by metaplasia and overgrowth of corneal endothelial cells. ZEB1 appears to be critical to epithelial to mesenchymal transitions (EMT) during development and cancer metastasis. As a transcription factor involved in EMT, ZEB1 promotes EMT by repressing genes associated with the epithelial phenotype and activates genes associated with the mesenchymal phenotype.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1074 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetZEB1
Antigen Species
Human
Gene ID6935
Gene Symbol
ZEB1
Gene Name
zinc finger E-box binding homeobox 1
Uniprot IDP37275
Protein Name
Zinc finger E-box-binding homeobox 1
Alternate Names
AREB6 • BZP • delta-crystallin enhancer binding factor 1 • DELTAEF1 • FECD6 • negative regulator of IL2 • NIL2A • NIL-2-A zinc finger protein • posterior polymorphous corneal dystrophy 3 • PPCD3 • TCF8 • TCF-8 • Transcription factor 8 • transcription factor 8 (represses interleukin 2 expression) • ZFHEP • ZFHX1A • zinc finger E-box-binding homeobox 1 • zinc finger homeodomain enhancer-binding protein
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZEB1 immobilized on solid support.
The epitope recognized by IHC-00419 maps to a region between residue 1074 and 1124 of human zinc finger E-box binding homeobox 1 using the numbering given in entry NP_110378.3 (GeneID 6935).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Documents
Safety Data SheetPDF · IHC-00419Open
Product DatasheetPDF · IHC-00419 · Lot 2 (current lot)Open
Product DatasheetPDF · IHC-00419 · Lot 2 (current lot)Open
Citations / Publications
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