Bethyl Laboratories
Rabbit anti-ZEB2/SIP IHC Antibody Affinity Purified
Primary
Catalog #IHC-00691
TargetZEB2/SIP
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
Image 1 of 2
local_shipping
Order within 20 hours and 53 minutes for shipment by Tuesday, November 10
Product Description
Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60315].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 750 and 800
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetZEB2/SIP
Antigen Species
Human
Gene ID9839
Gene Symbol
ZEB2
Gene Name
zinc finger E-box binding homeobox 2
Uniprot IDO60315
Protein Name
Zinc finger E-box-binding homeobox 2
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZEB2/SIP immobilized on solid support.
The epitope recognized by IHC-00691 maps to a region between residue 750 and 800 of human Smad interacting-protein 1 using the numbering given in entry NP_055610.1 (GeneID 9839).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00691Open
Product DatasheetPDF · IHC-00691 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00691 · Lot 1 (current lot)Open
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1