Bethyl Laboratories
Rabbit anti-ZNF198 Antibody Affinity Purified
Primary
Catalog #A301-710A
TargetZNF198
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
ZNF198 contains nine MYM-type zinc fingers. The N-terminal half of the ZNF198 gene has been identified as a gene fused to the cytoplasmic domain of the fibroblast growth factor receptor (FGFR1) gene as a result of a t(8;13) (p11;q11-12) chromosomal rearrangement. The t(8;13) chromosomal translocation is associated with myeloproliferative diseases such as stem cell leukemia lymphoma syndrome (SCLL). Studies of the normal function of ZNF198 have revealed that it associates with HHR6 and RAD18 and may be involved in the repair of DNA. ZNF198 has also been found to associate with the BHC110 subunit of a histone-deacetylase containing corepressor complex and may have an additional function in transcriptional repression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 975 and 1025
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetZNF198
Antigen Species
Human
Gene ID7750
Gene Symbol
ZMYM2
Gene Name
zinc finger MYM-type containing 2
Uniprot IDQ9UBW7
Protein Name
Zinc finger MYM-type protein 2
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZNF198 immobilized on solid support.
The epitope recognized by A301-710A maps to a region between residue 975 and 1025 of human zinc finger protein 198 using the numbering given in entry NP_003444.1 (GeneID 7750).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-710A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-710A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-710AOpen
Citations / Publications
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