Bethyl Laboratories
Rabbit anti-ZNF265/ZIS Antibody Affinity Purified
Primary
Catalog #A301-030A
TargetZNF265/ZIS
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
ZNF265 is an SR (serine/arginine-rich) domain containing zinc-finger protein and a component of the splicesome. ZNF265 interacts with U1-70K and U2AF35 and functions in directing alternative splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 270 and C-terminus of isoform 2
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetZNF265/ZIS
Antigen Species
Human
Gene ID9406
Gene Symbol
ZRANB2
Gene Name
zinc finger RANBP2-type containing 2
Uniprot IDO95218
Protein Name
Zinc finger Ran-binding domain-containing protein 2
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZNF265/ZIS immobilized on solid support.
The epitope recognized by A301-030A maps to a region between residue 270 and the C-terminus (residue 320) of human zinc-finger protein 265 (zinc-finger, splicing) using the numbering given in entry NP_005446.2 (GeneID 9406).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-030A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-030A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-030AOpen
Citations / Publications
Services
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