/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ZNF295 Antibody
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Primary Antibodies  

Rabbit anti-ZNF295 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

ZNF295

Reactivity:

Human

Applications:

IHC

,

IHC-IF

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 875 and 925

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ZNF295 immobilized on solid support.

The epitope recognized by A301-528A-T maps to a region between residue 875 and 925 of human zinc finger protein 295 using the numbering given in entry NP_065778.3 (GeneID 49854).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ZNF295 immobilized on solid support.

The epitope recognized by A301-528A maps to a region between residue 875 and 925 of human zinc finger protein 295 using the numbering given in entry NP_065778.3 (GeneID 49854).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Additional Product Information

ZNF295 bears 8 C2H2-type zinc fingers and a BTB/POZ (broad-complex, tramtrack, bric a brac/ Pox-virus and zinc finger) domain indicating a function in transcriptional regulation. ZNF295 has been shown to function as a transcriptional repressor and associates with ZFP161, a zinc-finger protein also know to function as a repressor of transcription.

49854

ZBTB21

zinc finger and BTB domain containing 21

Q9ULJ3

Zinc finger and BTB domain-containing protein 21

Alternate Names

zinc finger and BTB domain-containing protein 21; zinc finger protein 295; ZNF295

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.