Bethyl Laboratories
Rabbit anti-ZNF318/TZF Antibody Affinity Purified
Primary
Catalog #A301-545A
TargetZNF318/TZF
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
ZNF318/TZF is a Cys2-His2 type zinc-finger protein that has been found to function as a corepressor of the androgen receptor (AR). Co-repression by TZF is accomplished via the recruitment of histone deacetylase 2 to agonist-bound AR and the impairment of intranuclear foci formation by agonist-bound AR.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2075 and 2125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetZNF318/TZF
Antigen Species
Human
Gene ID24149
Gene Symbol
ZNF318
Gene Name
zinc finger protein 318
Uniprot IDQ5VUA4
Protein Name
Zinc finger protein 318
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZNF318/TZF immobilized on solid support.
The epitope recognized by A301-545A maps to a region between residue 1900 and 1950 of human zinc finger protein 318 (testicular zinc finger) using the numbering given in entry AAD17298.1 (GeneID 24149).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-545A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-545A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-545AOpen
Services
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