Bethyl Laboratories
Rabbit anti-ZNF638/NP220 Antibody, Affinity Purified
Primary
Catalog #A301-547A
TargetZNF638/NP220
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
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Product Description
ZNF638/NP220 is a Cys2-His2 zinc-finger DNA-binding protein that also bears repeats of an RNA recognition motif (RRM). ZNF638/NP220 has been reported to be structurally and functionally similar to matrin 3, a nuclear matrix protein involved in RNA processing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetZNF638/NP220
Antigen Species
Human
Gene ID27332
Gene Symbol
ZNF638
Gene Name
zinc finger protein 638
Uniprot IDQ14966
Protein Name
Zinc finger protein 638
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ZNF638/NP220 immobilized on solid support.
The epitope recognized by A301-547A maps to a region between residue 225 and 275 of human zinc finger protein 638 (Nuclear protein 220) using the numbering given in entry NP_055312.2 (GeneID 27332).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-547A · Lot 3 (current lot)Open
Product DatasheetPDF · A301-547A · Lot 3 (current lot)Open
Safety Data SheetPDF · A301-547AOpen
Services
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