Bethyl Laboratories
Sheep anti-Human Hemoglobin F Antibody Affinity Purified
Primary
Catalog #A80-136A
TargetHuman Hemoglobin F
HostSheep
ClonalityPolyclonal
ReactivityHuman
ApplicationELISA, WB
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Product Description
Hemoglobin is tetrameric protein that binds iron in individual heme groups. These molecules are responsible for the binding and transport of oxygen in the blood and are the most abundant component of red blood cells. Fetal hemoglobin binds oxygen with a higher affinity than adult hemoglobin.
Specifications
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Sheep were immunized with purified human hemoglobin F of fetal cord blood cell origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human hemoglobin F. No antibody was detected against hemoglobin A1, A2, S or human serum proteins.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A80-136AOpen
Product DatasheetPDF · A80-136A · Lot 230314 (current lot)Open
Safety Data SheetPDF · A80-136AOpen
Product DatasheetPDF · A80-136A · Lot 22Open
Safety Data SheetPDF · A80-136AOpen
Product DatasheetPDF · A80-136A · Lot 26Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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