Bethyl Laboratories
F(ab')2 Goat anti-Rabbit IgG-Fc Fragment Antibody Affinity Purified
Secondary
Catalog #A120-115A
TargetRabbit IgG-Fc Fragment
HostGoat
ClonalityPolyclonal
ReactivityRabbit
ApplicationELISA, ICC, IHC, WB
local_shipping
Order within 2 hours and 57 minutes for shipment by today
Product Description
IgG is the primary immunoglobulin protein found in blood/plasma, and functions to neutralize toxins, active complement, immobilizing pathogens and inducing opsonization, and cell-mediated cytotoxicity. The anti-Fc activity ensures activity only to the Fc portion of the IgG molecule and not the Fab fragments on the light chain.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Rabbit
Format
F(ab')2
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was solid phase adsorbed to ensure class specificity. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A120-115AOpen
Product DatasheetPDF · A120-115A · Lot 1 (current lot)Open
Safety Data SheetPDF · A120-115AOpen
Product DatasheetPDF · A120-115A · Lot 1 (current lot)Open
Safety Data SheetPDF · A120-115AOpen
Product DatasheetPDF · A120-115A · Lot 2Open
Safety Data SheetPDF · A120-115AOpen
Product DatasheetPDF · A120-115A · Lot 4Open
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1