Bethyl Laboratories
Rabbit anti-MED12 Antibody Affinity Purified
Primary
Catalog #A300-774A
TargetMED12
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
MED12 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. In this context, MED12 interaction with beta-catenin has been shown to be required for Wnt-signaling dependent transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2127 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMED12
Antigen Species
Human
Gene ID9968
Gene Symbol
MED12
Gene Name
mediator complex subunit 12
Uniprot IDQ93074
Protein Name
Mediator of RNA polymerase II transcription subunit 12
Alternate Names
activator-recruited cofactor 240 kDa component • ARC240 • CAG repeat protein 45 • CAGH45 • FGS1 • HOPA • human opposite paired • MED12S • Mediator complex subunit 12 • mediator of RNA polymerase II transcription subunit 12 • mediator of RNA polymerase II transcription, subunit 12 homolog • OHDOX • OKS • OPA1 • OPA-containing protein • putative mediator subunit 12 • thyroid hormone receptor-associated protein complex 230 kDa component • thyroid hormone receptor-associated protein, 230 kDa subunit • TNRC11 • TRAP230 • trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit) • trinucleotide repeat-containing gene 11 protein
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MED12 immobilized on solid support.
The epitope recognized by A300-774A maps to a region between residue 2127 and the C-terminus (residue 2177) of human Mediator of RNA Polymerase II Transcription, subunit 12 using the numbering given in entry NP_005111.2 (GeneID 9968).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A300-774A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-774A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-774AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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