Bethyl Laboratories
Rabbit anti-Lamin-A/C Antibody Affinity Purified
Primary
Catalog #A303-430A
TargetLamin-A/C
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationICC-IF, IHC, IP, WB
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Product Description
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin A/C plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P02545].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetLamin-A/C
Antigen Species
Human
Gene ID4000
Gene Symbol
LMNA
Gene Name
lamin A/C
Uniprot IDP02545
Protein Name
Prelamin-A/C
Alternate Names
70 kDa lamin • CDCD1 • CDDC • CMD1A • CMT2B1 • EMD2 • epididymis secretory sperm binding protein • FPL • FPLD • FPLD2 • HGPS • IDC • lamin • lamin A/C-like 1 • LDP1 • LFP • LGMD1B • LMN1 • LMNC • LMNL1 • MADA • mandibuloacral dysplasia type A • prelamin-A/C • PRO1 • renal carcinoma antigen NY-REN-32
Product Information
Applications
ICC-IF, IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Lamin-A/C immobilized on solid support.
The epitope recognized by A303-430A maps to a region between residue 1 and 50 of human Lamin A/Lamin C using the numbering given in entry NP_733821.1 (GeneID 4000).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-430A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-430A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-430AOpen
Citations / Publications
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