Bethyl Laboratories
Rabbit anti-CD45 Antibody Affinity Purified
Primary
Catalog #A304-376A
TargetCD45
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC, IP, WB
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Product Description
CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08575].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1256 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCD45
Antigen Species
Human
Gene ID5788
Gene Symbol
PTPRC
Gene Name
protein tyrosine phosphatase, receptor type C
Uniprot IDP08575
Protein Name
Receptor-type tyrosine-protein phosphatase C
Product Information
Applications
ICC-IF, IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CD45 immobilized on solid support.
The epitope recognized by A304-376A maps to a region between residue 1254 to 1304 of human CD45 Molecule using the numbering given in entry P08575.2 (GeneID 5788).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:2000 - 1:10,000
Immunoprecipitation (IP)6 µg/mg lysate
Immunocytochemistry-Immunofluoresence (ICC-IF)1:1000 - 1:5000. Acetone or Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunohistochemistry (IHC)1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Documents
Product DatasheetPDF · A304-376A · Lot 2 (current lot)Open
Product DatasheetPDF · A304-376A · Lot 2 (current lot)Open
Safety Data SheetPDF · A304-376AOpen
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