Bethyl Laboratories
Rabbit anti-CD45 Recombinant Monoclonal Antibody [BL-178-12C7]
Monoclonal Antibodies
Catalog #A700-012
TargetCD45
HostRabbit
CloneBL-178-12C7
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IHC-IF, IP, WB
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Product Description
CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08575].
Specifications
Clonality
Recombinant Monoclonal
Clone
BL-178-12C7
Host
Rabbit
Immunogen
Between 1256 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
50 µg/ml
Target Identity
TargetCD45
Antigen Species
Human
Gene ID5788
Gene Symbol
PTPRC
Gene Name
protein tyrosine phosphatase, receptor type C
Uniprot IDP08575
Protein Name
Receptor-type tyrosine-protein phosphatase C
Product Information
Applications
Flow Cyt, ICC, IHC, IHC-IF, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a synthetic peptide representing a region between residue 1250 to 1306 (C-terminus) of human CD45 Molecule according to the numbering given in entry NP_002829.3 (GeneID 5788).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-012 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-012 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-012 · Lot 200316Open
Safety Data SheetPDF · A700-012Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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