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Rabbit anti-CD45 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CD45
Reactivity:
Human
Platforms:
PhenoImager™ HT
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1256 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CD45 immobilized on solid support.

The epitope recognized by A304-376A maps to a region between residue 1254 to 1304 of human CD45 Molecule using the numbering given in entry P08575.2 (GeneID 5788).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CD45 immobilized on solid support.

The epitope recognized by A304-376A-T maps to a region between residue 1254 to 1304 of human CD45 Molecule using the numbering given in entry P08575.2 (GeneID 5788).
Country of Origin
USA

Additional Product Information

CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08575].

5788
PTPRC
protein tyrosine phosphatase, receptor type C
P08575
Receptor-type tyrosine-protein phosphatase C

Alternate Names

B220; CD antigen CD45; CD45; CD45 antigen; CD45R; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.