Bethyl Laboratories
Mouse anti-Cytokeratin 18 Monoclonal Antibody [LDK18]
Monoclonal Antibodies
Catalog #A500-035A
TargetCytokeratin 18
HostMouse
CloneLDK18
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, WB
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Product Description
Cytokeratin 18 is a type I intermediate filament. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. [taken from NCBI Entrez Gene (Gene ID: 3875)].
Specifications
Clonality
Monoclonal
Clone
LDK18
Host
Mouse
Immunogen
Between 380 and C-terminus
Isotype
IgG1
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetCytokeratin 18
Antigen Species
Human
Gene ID3875
Gene Symbol
KRT18
Gene Name
keratin 18
Uniprot IDP05783
Protein Name
Keratin, type I cytoskeletal 18
Alternate Names
Product Information
Applications
Flow Cyt, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Monoclonal antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 380 and the C-terminus (residue 430) of human cytokeratin 18 using the numbering given in entry NP_000215.1 (Gene ID 3875).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A500-035A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-035A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-035A · Lot 1 (current lot)Open
Safety Data SheetPDF · A500-035AOpen
Services
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