Bethyl Laboratories
Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR029F]
Monoclonal Antibodies
Catalog #A700-029
TargetCD86
HostRabbit
CloneBLR029F
ReactivityHuman
ApplicationFlow Cyt, ICC, IP, WB
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Product Description
CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. CD86 is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response [taken from NCBI Entrez Gene (Gene ID: 942)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR029F
Host
Rabbit
Immunogen
Residues 31-245 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetCD86
Antigen Species
Human
Gene ID942
Gene Symbol
CD86
Gene Name
CD86 molecule
Uniprot IDP42081
Protein Name
T-lymphocyte activation antigen CD86
Product Information
Applications
Flow Cyt, ICC, IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human CD86 (residues 31-245, NP_787058.4).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed and permeabilized cells were stained with 0.2 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-029 · Lot 2 (current lot)Open
Product DatasheetPDF · A700-029 · Lot 2 (current lot)Open
Safety Data SheetPDF · A700-029Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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