/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR030F]
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Primary Antibodies  

Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR030F]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CD86

Reactivity:

Human

Applications:

Flow Cyt

,

ICC

,

IHC

,

IP

,

WB

Platforms:

COMET™

,

PhenoImager™ HT

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR030F

Format:

Whole IgG

Immunogen:

Between 279 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

$127.00 $482.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a region between residue 300 and the C-terminus (residue 329) of human CD86 molecule using the numbering given in entry NP_787058.4 (GeneID 942).

Country of Origin

USA

Additional Product Information

CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. CD86 is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response [taken from NCBI Entrez Gene (Gene ID: 942)].

942

CD86

CD86 molecule

P42081

T-lymphocyte activation antigen CD86

Alternate Names

Activation B7-2 antigen; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD antigen CD86; CD28LG2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; FUN-1; LAB72; T-lymphocyte activation antigen CD86

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.