Bethyl Laboratories
Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F]
Monoclonal Antibodies
Catalog #A700-037
TargetMED1
HostRabbit
CloneBLR037F
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15648].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR037F
Host
Rabbit
Immunogen
Between 1531 and C-terminus
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetMED1
Antigen Species
Human
Gene ID5469
Gene Symbol
MED1
Gene Name
mediator complex subunit 1
Uniprot IDQ15648
Protein Name
Mediator of RNA polymerase II transcription subunit 1
Alternate Names
activator-recruited cofactor 205 kDa component • ARC205 • CRSP1 • CRSP200 • DRIP205 • DRIP230 • Mediator complex subunit 1 • mediator of RNA polymerase II transcription subunit 1 • p53 regulatory protein RB18A • PBP • peroxisome proliferator-activated receptor-binding protein • PPAR-binding protein • PPARBP • PPARGBP • RB18A • Thyroid receptor-interacting protein 2 • TRAP220 • TR-interacting protein 2 • TRIP2 • TRIP-2 • vitamin D receptor-interacting protein 230 kD
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 1550 and the C-terminus (residue 1581) of human Mediator complex subunit 1 using the numbering given in entry NP_004765.2 (GeneID 5469).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:20 to 1:200. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry (IHC)1:20 to 1:200. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-037 · Lot 2Open
Product DatasheetPDF · A700-037 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-037 · Lot 2Open
Product DatasheetPDF · A700-037 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-037 · Lot 2Open
Safety Data SheetPDF · A700-037Open
Citations / Publications
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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