Bethyl Laboratories
Rabbit anti-B7-H4 Recombinant Monoclonal Antibody [BLR043F]
Monoclonal Antibodies
Catalog #A700-043
TargetB7-H4
HostRabbit
CloneBLR043F
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IHC-IF, IP, WB
Image 1 of 6
local_shipping
Order within 11 hours and 35 minutes for shipment by Tuesday, November 10
Product Description
B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7-H4 plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P10914].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR043F
Host
Rabbit
Immunogen
Residues 30-282 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
50 µg/ml
Target Identity
TargetB7-H4
Antigen Species
Human
Gene ID79679
Gene Symbol
VTCN1
Gene Name
V-set domain containing T cell activation inhibitor 1
Uniprot IDQ7Z7D3
Protein Name
V-set domain-containing T-cell activation inhibitor 1
Alternate Names
Product Information
Applications
Flow Cyt, ICC, IHC, IHC-IF, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human B7-H4 (residues 30-282, NP_078902.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed and permeabilized cells were stained with 4 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-043 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-043 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-043 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-043Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1