Bethyl Laboratories
Rabbit anti-SOX10 Recombinant Monoclonal Antibody [BLR080G]
Monoclonal Antibodies
Catalog #A700-080-T
TargetSOX10
HostRabbit
CloneBLR080G
ReactivityMouse, Human
ApplicationICC, IHC, IP, mIF, WB
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Product Description
SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. SOX10 may act as a transcriptional activator after forming a protein complex with other proteins. SOX10 acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development [taken from NCBI Entrez Gene (Gene ID: 6663)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR080G
Host
Rabbit
Immunogen
Between 416 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
50 µg/ml
Target Identity
TargetSOX10
Antigen Species
Human
Gene ID6663
Gene Symbol
SOX10
Gene Name
SRY-box 10
Uniprot IDP56693
Protein Name
Transcription factor SOX-10
Alternate Names
Product Information
Applications
ICC, IHC, IP, mIF, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 416 and the C-terminus (residue 466) of human SOX10 using the numbering given in entry NP_008872.1 (Gene ID 6663).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Multiplex Immunofluorescence (mIF)1:250
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-080-T · Lot 1 (current lot)Open
Product DatasheetPDF · A700-080-T · Lot 200115Open
Safety Data SheetPDF · A700-080-TOpen
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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