Bethyl Laboratories
Rabbit anti-Cytokeratin 20 Recombinant Monoclonal Antibody [BLR105H]
Monoclonal Antibodies
Catalog #A700-105
TargetCytokeratin 20
HostRabbit
CloneBLR105H
ReactivityHuman
ApplicationICC, IHC, IP, WB
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Product Description
Cytokeratin 20 is a member of the keratin family of intermediate filament proteins responsible for the structural integrity of epithelial cells. Cytokeratin 20 is a type I cytokeratin, acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. [taken from NCBI Entrez Gene (Gene ID: 54474)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR105H
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
TargetCytokeratin 20
Antigen Species
Human
Gene ID54474
Gene Symbol
KRT20
Gene Name
keratin 20
Uniprot IDP35900
Protein Name
Keratin, type I cytoskeletal 20
Alternate Names
CD20 • CK20 • CK-20 • cytokeratin 20 • Cytokeratin-20 • K20 • keratin 20, type I • keratin, type I cytoskeletal 20 • Keratin-20 • KRT21 • protein IT
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 250 and 300 of human keratin, type I cytoskeletal 20 using the numbering given in entry NP_061883.1 (Gene ID 54474).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-105 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-105 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-105 · Lot 200116Open
Safety Data SheetPDF · A700-105Open
Services
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