Bethyl Laboratories
Rabbit anti-MCM5 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00041
TargetMCM5
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM5 can form a subcomplex with MCM3. MCM5 also appears to play a role in transcription. MCM5 has been shown to interact with the signal transducer and activator of transcription 1 (STAT1) and demonstrated to be essential for STAT1 target gene transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMCM5
Antigen Species
Human
Gene ID4174
Gene Symbol
MCM5
Gene Name
minichromosome maintenance complex component 5
Uniprot IDP33992
Protein Name
DNA replication licensing factor MCM5
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MCM5 immobilized on solid support.
The epitope recognized by IHC-00041 maps to a region between residue 1 and 50 of human Minichromosome Maintenance 5 using the numbering given in entry NP_006730.2 (GeneID 4174).
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00041Open
Product DatasheetPDF · IHC-00041 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00041 · Lot 1 (current lot)Open
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