Bethyl Laboratories
Rabbit anti-KRR1 Antibody Affinity Purified
Primary
Catalog #A303-898A
TargetKRR1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
KRR1 is required for 40S ribosome biogenesis. KRR1 is involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13601].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetKRR1
Antigen Species
Human
Gene ID11103
Gene Symbol
KRR1
Gene Name
KRR1, small subunit processome component homolog
Uniprot IDQ13601
Protein Name
KRR1 small subunit processome component homolog
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to KRR1 immobilized on solid support.
The epitope recognized by A303-898A maps to a region between residue 300 and 350 of human Small Subunit (SSU) Processome Component using the numbering given in entry NP_008974.5 (GeneID 11103).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-898A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-898A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-898AOpen
Citations / Publications
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