Bethyl Laboratories
Rabbit anti-Calpastatin/CAST Antibody Affinity Purified
Primary
Catalog #A304-794A
TargetCalpastatin/CAST
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation [taken from NCBI Entrez Gene (Gene ID: 831)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 658 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCalpastatin/CAST
Antigen Species
Human
Gene ID831
Gene Symbol
CAST
Gene Name
calpastatin
Uniprot IDP20810
Protein Name
Calpastatin
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Calpastatin/CAST immobilized on solid support.
The epitope recognized by A304-794A maps to a region between residue 658 to 708 of human Calpastatin using the numbering given in entry P20810 (GeneID 831).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-794A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-794A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-794AOpen
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