Bethyl Laboratories
Rabbit anti-AKT1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00513
TargetAKT1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
AKT1 is a serine-threonine kinase that functions downstream of PI-3 kinase and is known to phosphorylate several proteins. AKT1 mediates the effects of several growth factors such as insulin, IGF-1, EGF, and PDGF to control cell survival and apoptosis. There are numerous substrates of AKT1, some of which are: GSK3-alpha and beta, mTOR, TSC2, Bad, c-Raf, and caspase 9.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetAKT1
Antigen Species
Human
Gene ID207
Gene Symbol
AKT1
Gene Name
AKT serine/threonine kinase 1
Uniprot IDP31749
Protein Name
RAC-alpha serine/threonine-protein kinase
Alternate Names
AKT • AKT1m • CWS6 • PKB • PKB alpha • PKB-ALPHA • PRKBA • Protein kinase B • protein kinase B alpha • proto-oncogene c-Akt • RAC • rac protein kinase alpha • RAC-ALPHA • RAC-alpha serine/threonine-protein kinase • RAC-PK-alpha • serine-threonine protein kinase • v-akt murine thymoma viral oncogene homolog 1 • v-akt murine thymoma viral oncogene-like protein 1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AKT1 immobilized on solid support.
The epitope recognized by IHC-00513 maps to a region between residue 25 and 75 of human v-akt murine thymoma viral oncogene homolog 1 using the numbering given in entry NP_005154.1 (GeneID 207).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00513Open
Product DatasheetPDF · IHC-00513 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00513 · Lot 1 (current lot)Open
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