Bethyl Laboratories

Rabbit anti-GORAB Antibody Affinity Purified

Primary
Catalog #A305-691A
TargetGORAB
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human GORAB by western blot of immunoprecipitates.
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Product Description

GORAB is a member of the golgin family, a group of coiled-coil proteins localized to the Golgi. GORAB may function in the secretory pathway. GORAB localizes to the cytoplasm and was identified by interactions with the N-terminal kinase-like protein. It may function in mitosis [taken from NCBI Entrez Gene (Gene ID: 92344)].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 50 and 100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGORAB
Antigen Species
Human
Gene ID92344
Gene Symbol
GORAB
Gene Name
golgin, RAB6 interacting
Uniprot IDQ5T7V8
Protein Name
RAB6-interacting golgin
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to GORAB immobilized on solid support.

The epitope recognized by A305-691A maps to a region between residue 75 to 125 of human RAB6-interacting golgin using the numbering given in entry Q5T7V8.1 (GeneID 92344).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A305-691A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-691A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-691AOpen
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