/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-B7-H4 Recombinant Monoclonal Antibody [BLR043F]
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Primary Antibodies  

Rabbit anti-B7-H4 Recombinant Monoclonal Antibody [BLR043F]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

B7-H4

Reactivity:

Human

Applications:

Flow Cyt

,

ICC

,

IHC

,

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR043F

Format:

Whole IgG

Immunogen:

Residues 30-282 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
50 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing the extracellular domain of human B7-H4 (residues 30-282, NP_078902.2).

Country of Origin

USA

Additional Product Information

B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7-H4 plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P10914].

79679

VTCN1

V-set domain containing T cell activation inhibitor 1

Q7Z7D3

V-set domain-containing T-cell activation inhibitor 1

Alternate Names

B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7h.5; B7H4; B7-H4; B7S1; B7X; immune costimulatory protein B7-H4; PRO1291; Protein B7S1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; VCTN1; V-set domain-containing T-cell activation inhibitor 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.