Bethyl Laboratories
Rabbit anti-BAF57/SMARCE1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00129
TargetBAF57/SMARCE1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. BAF57/SMARCE1 is a core subunit found in all SWI/SNF complexes. The BAF57/SMARCE1 subunit has been demonstrated to interact with the estrogen receptor (ER) and is required for the coactivation of ER-mediated gene transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 361 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetBAF57/SMARCE1
Antigen Species
Human
Gene ID6605
Gene Symbol
SMARCE1
Gene Name
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Uniprot IDQ969G3
Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BAF57/SMARCE1 immobilized on solid support.
The epitope recognized by IHC-00129 maps to a region between residue 360 and the C-terminus (residue 411) of human BRG1-Associated Factor 57 (SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin E1) using the numbering given in entry NP_003070.3 (GeneID 6605).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00129Open
Product DatasheetPDF · IHC-00129 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00129 · Lot 1 (current lot)Open
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