Bethyl Laboratories
Rabbit anti-BIG2/ARFGEF2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00545
TargetBIG2/ARFGEF2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
BIG2/ARFGEF2 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetBIG2/ARFGEF2
Antigen Species
Human
Gene ID10564
Gene Symbol
ARFGEF2
Gene Name
ADP ribosylation factor guanine nucleotide exchange factor 2
Uniprot IDQ9Y6D5
Protein Name
Brefeldin A-inhibited guanine nucleotide-exchange protein 2
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BIG2/ARFGEF2 immobilized on solid support.
The epitope recognized by IHC-00545 maps to a region between residue 300 and 350 of human brefeldin A-inhibited 2 (ADP-ribosylation factor guanine nucleotide-exchange factor 2) using the numbering given in entry NP_006411.1 (GeneID 10564).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00545Open
Product DatasheetPDF · IHC-00545 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00545 · Lot 1 (current lot)Open
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