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Rabbit anti-BMI1 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
BMI1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 276 and 326
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine, Chicken, Orangutan, Cat
Antigen Species
Human
Stock Concentration
100 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to BMI1 immobilized on solid support.

The epitope recognized by A301-694A-T maps to a region between residue 276 and 326 of human B lymphoma Mo-MLV insertion region 1 homolog using the numbering given in entry NP_005171.4 (GeneID 648).

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Gene & Protein Information

BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) is a core component of the polycomb group (PcG) multiprotein PRC1 complex which plays an important role in transcriptional repression of gene expression during development. BMI1 association with RING protein 1B is important to the E3 ligase activity of RING1B in the PRC1 complex. BMI1 is an oncogene whose overexpression is associated with many types of human tumors and is a stem-cell marker and regulator of stem cell self-renewal.

648

BMI1

BMI1 proto-oncogene, polycomb ring finger

P35226

Polycomb complex protein BMI-1

Alternate Names

B lymphoma Mo-MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, BMI1 polycomb ring finger proto-oncogene, FLVI2/BMI1, flvi-2/bmi-1, murine leukemia viral (bmi-1) oncogene homolog, PCGF4, polycomb complex protein BMI-1, polycomb group protein Bmi1, polycomb group RING finger protein 4, ring finger protein 51, RNF51

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Citations (6)