Bethyl Laboratories
Rabbit anti-BMI1 Antibody Affinity Purified
Primary
Catalog #A301-694A
TargetBMI1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) is a core component of the polycomb group (PcG) multiprotein PRC1 complex which plays an important role in transcriptional repression of gene expression during development. BMI1 association with RING protein 1B is important to the E3 ligase activity of RING1B in the PRC1 complex. BMI1 is an oncogene whose overexpression is associated with many types of human tumors and is a stem-cell marker and regulator of stem cell self-renewal.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 276 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetBMI1
Antigen Species
Human
Gene ID648
Gene Symbol
BMI1
Gene Name
BMI1 proto-oncogene, polycomb ring finger
Uniprot IDP35226
Protein Name
Polycomb complex protein BMI-1
Alternate Names
B lymphoma Mo-MLV insertion region 1 homolog • BMI1 polycomb ring finger oncogene • BMI1 polycomb ring finger proto-oncogene • FLVI2/BMI1 • flvi-2/bmi-1 • murine leukemia viral (bmi-1) oncogene homolog • PCGF4 • polycomb complex protein BMI-1 • polycomb group protein Bmi1 • polycomb group RING finger protein 4 • ring finger protein 51 • RNF51
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BMI1 immobilized on solid support. The epitope recognized by A301-694A maps to a region between residue 276 and 326 of human B lymphoma Mo-MLV insertion region 1 homolog using the numbering given in entry NP_005171.4 (GeneID 648).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-694A · Lot 4 (current lot)Open
Product DatasheetPDF · A301-694A · Lot 4 (current lot)Open
Safety Data SheetPDF · A301-694AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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