Bethyl Laboratories
Rabbit anti-BMI1 Recombinant Monoclonal Antibody [BLR119H]
Monoclonal Antibodies
Catalog #A700-119
TargetBMI1
HostRabbit
CloneBLR119H
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) is a core component of the polycomb group (PcG) multiprotein PRC1 complex which plays an important role in transcriptional repression of gene expression during development. BMI1 association with RING protein 1B is important to the E3 ligase activity of RING1B in the PRC1 complex. BMI1 is an oncogene whose overexpression is associated with many types of human tumors and is a stem-cell marker and regulator of stem cell self-renewal.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR119H
Host
Rabbit
Immunogen
Between 623 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetBMI1
Antigen Species
Human
Gene ID648
Gene Symbol
BMI1
Gene Name
BMI1 proto-oncogene, polycomb ring finger
Uniprot IDP35226
Protein Name
Polycomb complex protein BMI-1
Alternate Names
B lymphoma Mo-MLV insertion region 1 homolog • BMI1 polycomb ring finger oncogene • BMI1 polycomb ring finger proto-oncogene • FLVI2/BMI1 • flvi-2/bmi-1 • murine leukemia viral (bmi-1) oncogene homolog • PCGF4 • polycomb complex protein BMI-1 • polycomb group protein Bmi1 • polycomb group RING finger protein 4 • ring finger protein 51 • RNF51
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 250 and 300 of human polycomb complex protein BMI1 using the numbering given in entry NP_005171.4 (Gene ID 648).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-119 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-119 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-119Open
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Resources
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Immunoprecipitation Protocol
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