Bethyl Laboratories
Rabbit anti-BRD7 Antibody Affinity Purified
Primary
Catalog #A302-304A
TargetBRD7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationFlow Cyt, IHC, IP, WB
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Product Description
Bromodomain-containing protein 7 was identified in a two-hybrid screen as an interactor of interferon regulatory factor-2 (IRF-2) and called CELTIX1. BRD7 was also identified as a binding partner of disheveled-1 (Dvl-1) and found to play a role in Wnt signaling and the regulation of glycogen synthase kinase 3-beta activity. Recently, BRD7 was identified as a subunit of the polybromo-associated BRG1-associated factor (PBAF) complex and shown to be required for the modulation of transcription in embryonic stem cells.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 601 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetBRD7
Antigen Species
Human
Gene ID29117
Gene Symbol
BRD7
Gene Name
bromodomain containing 7
Uniprot IDQ9NPI1
Protein Name
Bromodomain-containing protein 7
Alternate Names
Product Information
Applications
Flow Cyt, IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to BRD7 immobilized on solid support.
The epitope recognized by A302-304A maps to a region between residue 601 and 651 of human bromodomain containing 7 using the numbering given in entry NP_037395.2 (GeneID 29117).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A302-304A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-304A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-304AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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