Bethyl Laboratories
Rabbit anti-BRD7 Recombinant Monoclonal Antibody [BLR220K]
Monoclonal Antibodies
Catalog #A700-220
TargetBRD7
HostRabbit
CloneBLR220K
ReactivityMouse, Human
ApplicationFlow Cyt, WB
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Product Description
Bromodomain-containing protein 7 was identified in a two-hybrid screen as an interactor of interferon regulatory factor-2 (IRF-2) and called CELTIX1. BRD7 was also identified as a binding partner of disheveled-1 (Dvl-1) and found to play a role in Wnt signaling and the regulation of glycogen synthase kinase 3-beta activity. Recently, BRD7 was identified as a subunit of the polybromo-associated BRG1-associated factor (PBAF) complex and shown to be required for the modulation of transcription in embryonic stem cells.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR220K
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
500 µg/ml
Target Identity
TargetBRD7
Antigen Species
Human
Gene ID29117
Gene Symbol
BRD7
Gene Name
bromodomain containing 7
Uniprot IDQ9NPI1
Protein Name
Bromodomain-containing protein 7
Alternate Names
Product Information
Applications
Flow Cyt, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 150 and 200 of human Bromodomain-containing protein 7 using the numbering given in entry NP_037395.2 (Gene ID 29117).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-220 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-220 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-220 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-220Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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