Bethyl Laboratories
Rabbit anti-c-Maf Antibody Affinity Purified
Primary
Catalog #A300-613A
Targetc-Maf
HostRabbit
ClonalityPolyclonal
ReactivityMouse
ApplicationWB
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Product Description
c-Maf is part of the Maf family of transcription factors that possess a conserved basic region and a leucine zipper domain that mediate DNA and protein-protein binding and plays an important role in tissue-specific gene regulation and cell differentiation. c-Maf is a transcriptional activator of interleukin 4 and 10 in T-cells, and is involved in lens fiber cell differentiation. c-Maf translocation has been observed in human multiple myeloma and may be oncogenic.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Targetc-Maf
Antigen Species
Mouse
Gene ID17132
Gene Symbol
Maf
Gene Name
avian musculoaponeurotic fibrosarcoma oncogene homolog
Uniprot IDP54843
Protein Name
Transcription factor Maf
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
The epitope recognized by A300-613A maps to a region between residue 150 and 200 of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog (c-Maf) using the numbering given in entry NP_001020748. 2 (GeneID 17132).
Antibody was affinity purified using the peptide immobilized on solid support.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-613A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-613A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-613AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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